The Human PDCN (Podocin) ELISA Kit is specifically designed for the precise measurement of podocin levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Podocin is a critical protein that plays a key role in maintaining the integrity of the kidney filtration barrier. Dysregulation of podocin is associated with various kidney diseases, including nephrotic syndrome and diabetic nephropathy.
Therefore, the Human PDCN ELISA Kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Human PDCN ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for studying podocin levels in biological samples, offering valuable insights into kidney function and related diseases.
Product Name:
Human PDCN (Podocin) ELISA Kit
SKU:
HUES02037
Target:
Human PDCN (Podocin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDCN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDCN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDCN. You can calculate the concentration of Human PDCN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
96-114
98-109
Average (%)
94
104
104
1:4
Range (%)
89-99
85-96
87-103
Average (%)
94
90
94
1:8
Range (%)
89-100
87-98
86-102
Average (%)
95
92
93
1:16
Range (%)
91-107
80-92
89-102
Average (%)
97
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.58
7.62
1.13
2.48
7.34
Standard deviation
0.07
0.12
0.33
0.06
0.14
0.27
C V (%)
6.42
4.65
4.33
5.31
5.65
3.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDCN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDCN in samples. No significant cross-reactivity or interference between Human PDCN and analogues was observed.
