Human PDGF-AB (Platelet Derived Growth Factor AB) CLIA Kit
The Human PDGF (Platelet-Derived Growth Factor) AB CLIA Kit is specifically designed for the accurate measurement of PDGF levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.PDGF is a key growth factor that plays a critical role in the regulation of cell growth and division, particularly in processes such as wound healing and tissue repair.
Abnormal PDGF levels have been implicated in various diseases, including cancer, fibrosis, and vascular disorders, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.With its advanced technology and reliable performance, the Human PDGF AB CLIA Kit is an indispensable tool for researchers studying PDGF biology and its implications in human health and disease. Get your kit today and unlock the potential for groundbreaking discoveries.
Product Name:
Human PDGF-AB (Platelet Derived Growth Factor AB) CLIA Kit
SKU:
HUES00893
Target:
Human PDGF-AB (Platelet Derived Growth Factor AB)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-AB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-AB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-AB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDGF-AB. You can calculate the concentration of Human PDGF-AB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-109
90-102
Average (%)
97
99
96
1:4
Range (%)
102-115
98-113
99-113
Average (%)
108
105
105
1:8
Range (%)
96-112
90-101
101-119
Average (%)
104
95
109
1:16
Range (%)
96-110
101-119
99-113
Average (%)
103
109
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.85
131.52
404.3
53.7
136.51
398.26
Standard deviation
4.78
9.68
24.54
5.66
11.34
44.68
C V (%)
9.22
7.36
6.07
10.54
8.31
11.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDGF-AB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGF-AB in samples. No significant cross-reactivity or interference between Human PDGF-AB and analogues was observed.
