The Human Pentosidine ELISA Kit is specifically designed for the precise measurement of pentosidine levels in human biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it a valuable tool for various research applications. Pentosidine is a key advanced glycation end product (AGE) known to play a significant role in the pathogenesis of various chronic diseases, including diabetes, cardiovascular diseases, and osteoporosis. By accurately quantifying pentosidine levels, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic interventions.
This ELISA kit offers an efficient and convenient method for detecting and quantifying pentosidine levels, enabling researchers to advance their understanding of the role of glycation in disease progression. By using this kit, researchers can confidently study the impact of pentosidine on disease development and progression, paving the way for innovative treatment strategies.
Product Name:
Human Pentosidine ELISA Kit
SKU:
HUFI02800
Reactivity:
Human
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
3.75 ng/mL
Range:
6.25-400 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
89-99%
89-95%
81-89%
EDTA Plasma (n = 10)
91-100%
87-99%
81-96%
Heparin Plasma (n = 10)
86-101%
86-98%
81-92%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
90-103
95
EDTA Plasma (n = 10)
88-101
92
Heparin Plasma (n = 10)
91-104
97
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
