The Human PGRN (Progranulin) CLIA Kit is specifically engineered for the precise measurement of progranulin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for a variety of research applications.Progranulin is a key protein involved in various biological processes, including inflammation, wound healing, and neurodegeneration. Dysregulation of progranulin has been linked to conditions such as neurodegenerative diseases, autoimmune disorders, and cancer, highlighting its significance as a potential biomarker for disease progression and therapeutic development.
With its advanced technology and superior performance, the Human PGRN CLIA Kit is an indispensable resource for researchers studying the role of progranulin in health and disease, providing valuable insights and opportunities for innovative research advancements.
Product Name:
Human PGRN (Progranulin) CLIA Kit
SKU:
HUES00895
Target:
Human PGRN (Progranulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PGRN. You can calculate the concentration of Human PGRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
91-103
97-109
Average (%)
98
97
103
1:4
Range (%)
95-112
90-102
93-107
Average (%)
103
96
99
1:8
Range (%)
86-98
95-109
89-102
Average (%)
93
102
95
1:16
Range (%)
87-103
92-103
100-119
Average (%)
94
97
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
1.85
7.92
0.94
1.98
8.59
Standard deviation
0.08
0.18
0.71
0.1
0.19
0.73
C V (%)
8.42
9.73
8.96
10.64
9.6
8.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PGRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed.
