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Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA Kit (SBRS1877)

SKU:
SBRS1877
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
€820
Frequently bought together:

Description

Human Phospho-LAT (Y161) and Total LAT PharmaGenie ELISA Kit (SBRS1877)

Assay Genie Phospho-LAT (Tyr161) and Total LAT ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated LAT protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-LAT and total LAT. An anti-pan LAT antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and LAT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and mouse anti-LAT (Tyr161) antibody is used to detect phosphorylated LAT and pan LAT. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LAT (Tyr161) or pan LAT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.