Human PIIINP (N-Terminal Procollagen 3 Propeptide) CLIA Kit
The Human PIIINP (N-terminal propeptide of type III procollagen) CLIA Kit is a reliable and accurate assay for the quantitative measurement of PIIINP levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing researchers with dependable and reproducible results for a variety of research applications.PIIINP is a key biomarker associated with collagen synthesis and tissue remodeling, making it a valuable tool for studying conditions such as fibrosis, liver disease, and bone metabolism.
Understanding PIIINP levels can provide insight into disease progression and treatment outcomes, making this CLIA kit an essential component in biomarker research and therapeutic development.
Product Name:
Human PIIINP (N-Terminal Procollagen 3 Propeptide) CLIA Kit
SKU:
HUES00157
Target:
Human PIIINP (N-Terminal Procollagen 3 Propeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Pâ…¢NP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pâ…¢NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pâ…¢NP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Pâ…¢NP. You can calculate the concentration of Human Pâ…¢NP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
94-107
99-115
Average (%)
94
102
106
1:4
Range (%)
86-96
96-112
88-102
Average (%)
91
103
94
1:8
Range (%)
93-110
89-101
96-112
Average (%)
101
95
104
1:16
Range (%)
89-105
99-116
85-95
Average (%)
96
107
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
98-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.9
72.65
396.54
40.81
77.7
365.7
Standard deviation
4.22
6.84
42.87
3.86
5.94
28.96
C V (%)
10.85
9.42
10.81
9.46
7.64
7.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Pâ…¢NP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pâ…¢NP in samples. No significant cross-reactivity or interference between Human Pâ…¢NP and analogues was observed.
