Human PPP1CC Recombinant Protein (RPPB2164)
- SKU:
- RPPB2164
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P36873
- Research Area:
- Enzymes
Description
| Product Name: | Human PPP1CC Recombinant Protein |
| Product Code: | RPPB2164 |
| Size: | 5µg |
| Species: | Human |
| Target: | PPP1CC |
| Synonyms: | Protein phosphatase 1 catalytic subunit gamma isozyme/isoform, Protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G, EC 3.1.3.16. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PPP1CC protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKK |
| Biological Activity: | Specific activity is > 700 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37�C. |
PPP1CC is vital for cell division and takes part in the regulation of protein synthesis, muscle contractility and glycogen metabolism. Additionally, PPP1CC has a role in long-term synaptic plasticity and regulation of ionic conductance, and has a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
PPP1CC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-323a.a.) and having a molecular mass of 39.1kDa.PPP1CC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPP1CC: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma. |
| UniProt Protein Details: | Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus Chromosomal Location of Human Ortholog: 12q24.1-q24.2 Cellular Component: focal adhesion; protein complex; mitochondrion; cytoplasm; nucleolus; nuclear speck; midbody; cytosol; nucleus; cleavage furrow Molecular Function:protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding Biological Process: glycogen metabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; mitotic cell cycle; circadian regulation of gene expression; regulation of circadian rhythm; protein amino acid dephosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; entrainment of circadian clock by photoperiod |
| NCBI Summary: | The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P36873 |
| NCBI GenInfo Identifier: | 548573 |
| NCBI Gene ID: | 5501 |
| NCBI Accession: | P36873.1 |
| UniProt Related Accession: | P36873 |
| Molecular Weight: | 323 |
| NCBI Full Name: | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit |
| NCBI Synonym Full Names: | protein phosphatase 1, catalytic subunit, gamma isozyme |
| NCBI Official Symbol: | PPP1CC�� |
| NCBI Official Synonym Symbols: | PP1C; PP-1G; PPP1G�� |
| NCBI Protein Information: | serine/threonine-protein phosphatase PP1-gamma catalytic subunit; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform |
| UniProt Protein Name: | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit |
| UniProt Synonym Protein Names: | Protein phosphatase 1C catalytic subunit |
| UniProt Gene Name: | PPP1CC�� |
| UniProt Entry Name: | PP1G_HUMAN |
