Human PTF1 alpha (Pancreas Specific Transcription Factor 1 alpha) CLIA Kit
The Human PTF1-alpha (Pancreas Specific Transcription Factor 1-alpha) CLIA Kit is designed for the sensitive and specific detection of PTF1-alpha levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides accurate and reliable results, making it suitable for a variety of research applications.PTF1-alpha is a key transcription factor involved in pancreatic development and function. It plays a crucial role in regulating gene expression in pancreatic cells and is essential for proper pancreas formation.
Dysregulation of PTF1-alpha has been linked to pancreatic disorders and diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its high sensitivity and specificity, the Human PTF1-alpha CLIA Kit is a valuable tool for researchers studying pancreatic biology, disease mechanisms, and potential therapeutic interventions. Get precise and reproducible results with this advanced kit from Assay Genie.
Product Name:
Human PTF1 alpha (Pancreas Specific Transcription Factor 1 alpha) CLIA Kit
SKU:
HUES01173
Target:
Human PTF1 alpha (Pancreas Specific Transcription Factor 1 alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTF1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTF1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTF1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PTF1α. You can calculate the concentration of Human PTF1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
103-119
99-113
Average (%)
105
109
106
1:4
Range (%)
101-117
93-104
91-106
Average (%)
107
99
98
1:8
Range (%)
103-117
99-114
95-110
Average (%)
109
104
101
1:16
Range (%)
101-113
94-107
90-105
Average (%)
106
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
102-115
108
Cell culture media (n=5)
98-110
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.88
275.43
987.0
98.76
274.35
1044.15
Standard deviation
8.84
32.09
104.03
8.71
21.87
109.22
C V (%)
8.12
11.65
10.54
8.82
7.97
10.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PTF1α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTF1α in samples. No significant cross-reactivity or interference between Human PTF1α and analogues was observed.
