The Human PYY (Peptide YY) ELISA Kit is specifically designed for the precise quantification of peptide YY levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes. Peptide YY is a key peptide hormone that plays a crucial role in regulating appetite and food intake. It is involved in the control of energy homestasis and has been implicated in various metabolic disorders such as obesity and diabetes. Thus, the measurement of peptide YY levels is essential for understanding the mechanisms underlying these conditions and exploring potential therapeutic interventions. With its advanced technology and reliable performance, the Human PYY ELISA Kit from Assay Genie provides researchers with a valuable tool for studying the physiological and pathological functions of peptide YY in both basic and clinical research settings.
Product Name:
Human PYY (Peptide YY) ELISA Kit
SKU:
HUES02302
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
