Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
The Assay Genie Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit has been meticulously crafted for the precise quantification of RANTES levels in diverse biological samples. RANTES, known as Regulated On Activation, Normal T-Cell Expressed and Secreted, is a pivotal chemokine involved in immune regulation, inflammation, and the recruitment of immune cells to sites of inflammation or infection. By accurately measuring RANTES levels, researchers can gain valuable insights into the immune response, inflammatory processes, and various immune-mediated disorders, enabling the development of targeted therapeutic interventions in these essential areas.
Our RANTES ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your scientific investigations. Manufactured under stringent quality control measures, this kit delivers reliable performance and user-friendly procedures, making it a preferred choice for conducting impactful research in immunology and related fields. Trust Assay Genie's RANTES ELISA Kit for dependable and precise quantification, empowering your exploration into the roles of this critical chemokine.
Product Name:
Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
SKU:
AEES00179
Target:
Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RANTES. You can calculate the concentration of Human RANTES in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
98-112
97-111
Average (%)
101
104
104
1:4
Range (%)
96-111
96-113
97-110
Average (%)
103
104
104
1:8
Range (%)
92-105
94-107
94-109
Average (%)
99
100
102
1:16
Range (%)
91-107
94-108
99-114
Average (%)
99
101
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.67
585.2
2254.99
253.11
557.11
2344.96
Standard deviation
17.31
23.47
97.19
14.2
29.81
80.2
C V (%)
6.64
4.01
4.31
5.61
5.35
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RANTES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RANTES in samples. No significant cross-reactivity or interference between Human RANTES and analogues was observed.
