Description
| Product Name: | Human RFXANK Recombinant Protein |
| Product Code: | RPPB4445 |
| Size: | 10µg |
| Species: | Human |
| Target: | RFXANK |
| Synonyms: | ANKRA1, BLS, F14150_1, RFX-B, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFXANK, RFXB. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RFXANK solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE |
Regulatory Factor X-Associated Ankyrin-Containing Protein (RFXANK) contains ankyrin repeats involved in protein-protein interactions. RFXANK, along with regulatory factor X-associated protein and regulatory factor-5, figures a complex which binds to the X box motif of certain MHC class II gene promoters and activates their transcription. After bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in RFXANK have been linked to bare lymphocyte syndrome type II, complementation group B.
RFXANK Human Recombinant produced in E. coli is a single polypeptide chain containing 260 amino acids (1-237) and having a molecular mass of 28kDa. RFXANK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RFXANK: Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. Defects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-binding; Transcription, coactivator/corepressorChromosomal Location of Human Ortholog: 19p12Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleusMolecular Function: transcription cofactor activityBiological Process: regulation of transcription from RNA polymerase II promoterDisease: Bare Lymphocyte Syndrome, Type Ii |
| UniProt Protein Details: | |
| NCBI Summary: | Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O14593 |
| NCBI GenInfo Identifier: | 6093962 |
| NCBI Gene ID: | 8625 |
| NCBI Accession: | O14593.2 |
| UniProt Secondary Accession: | O14593,O95839, Q24JQ1, Q6FGA8 |
| UniProt Related Accession: | O14593 |
| Molecular Weight: | 25,652 Da |
| NCBI Full Name: | DNA-binding protein RFXANK |
| NCBI Synonym Full Names: | regulatory factor X associated ankyrin containing protein |
| NCBI Official Symbol: | RFXANK�� |
| NCBI Official Synonym Symbols: | BLS; RFX-B; ANKRA1; F14150_1�� |
| NCBI Protein Information: | DNA-binding protein RFXANK |
| UniProt Protein Name: | DNA-binding protein RFXANK |
| UniProt Synonym Protein Names: | Ankyrin repeat family A protein 1; Regulatory factor X subunit B; RFX-B; Regulatory factor X-associated ankyrin-containing protein |
| Protein Family: | DNA-binding protein |
| UniProt Gene Name: | RFXANK�� |
| UniProt Entry Name: | RFXK_HUMAN |
