Description
| Product Name: | Human RGS4 Recombinant Protein |
| Product Code: | RPPB4455 |
| Size: | 10µg |
| Species: | Human |
| Target: | RGS4 |
| Synonyms: | Regulator of G-protein signaling 4, RGP4, RGS4, SCZD9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RGS4 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 10% glycerol and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHNSSHN KKDKVVICQR VSQEEVKKWA ESLENLISHE CGLAAFKAFL KSEYSEENID FWISCEEYKK IKSPSKLSPK AKKIYNEFIS VQATKEVNLD SCTREETSRN MLEPTITCFD EAQKKIFNLM EKDSYRRFLK SRFYLDLVNP SSCGAEKQKG AKSSADCASL VPQCA |
Regulator of G protein signaling 4 (RGS4) is a protein which regulates G protein signaling. Regulator of G protein signaling (RGS) family members are regulatory molecules which act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. Heterotrimeric G proteins serve to convey information from cell surface receptors to intracellular effectors.
RGS4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.4kDa.RGS4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GAPs, RGS; GAPs Chromosomal Location of Human Ortholog: 1q23.3 Cellular Component: cytoplasm; nucleus; plasma membrane Molecular Function:calmodulin binding Biological Process: inactivation of MAPK activity; regulation of G-protein coupled receptor protein signaling pathway Disease: Schizophrenia 9 |
| NCBI Summary: | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49798 |
| NCBI GenInfo Identifier: | 1710146 |
| NCBI Gene ID: | 5999 |
| NCBI Accession: | P49798.1 |
| UniProt Secondary Accession: | P49798,A7XA56, A7XA58, A7XA59, A7YVV7, B1APZ3, |
| UniProt Related Accession: | P49798 |
| Molecular Weight: | 21,452 Da |
| NCBI Full Name: | Regulator of G-protein signaling 4 |
| NCBI Synonym Full Names: | regulator of G-protein signaling 4 |
| NCBI Official Symbol: | RGS4�� |
| NCBI Official Synonym Symbols: | RGP4; SCZD9�� |
| NCBI Protein Information: | regulator of G-protein signaling 4 |
| UniProt Protein Name: | Regulator of G-protein signaling 4 |
| Protein Family: | Regulator of G-protein signaling |
| UniProt Gene Name: | RGS4�� |
| UniProt Entry Name: | RGS4_HUMAN |
