Human RNASE3 Recombinant Protein (RPPB2246)
- SKU:
- RPPB2246
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P12724
- Research Area:
- Enzymes
Description
| Product Name: | Human RNASE3 Recombinant Protein |
| Product Code: | RPPB2246 |
| Size: | 20µg |
| Species: | Human |
| Target: | RNASE3 |
| Synonyms: | ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RNASE3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I |
Ribonuclease 3 (RNASE3) is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. RNASE3 possesses a broad diversity of biological activities. RNASE3 protein has shown antibacterial activity such as cytoplasmic membrane depolarization of preferentially Gram-negative and Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
RNASE3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (28-160) and having a molecular mass of 19.9kDa. RNASE3 is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; EC 3.1.27.-; Ribonuclease Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular space; extracellular region Molecular Function:nucleic acid binding; endonuclease activity; ribonuclease activity Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response |
| NCBI Summary: | The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014] |
| UniProt Code: | P12724 |
| NCBI GenInfo Identifier: | 147744558 |
| NCBI Gene ID: | 6037 |
| NCBI Accession: | P12724.2 |
| UniProt Secondary Accession: | P12724,Q4VBC1, Q8WTP7, Q8WZ62, Q9GZN9, |
| UniProt Related Accession: | P12724 |
| Molecular Weight: | 18kDa |
| NCBI Full Name: | Eosinophil cationic protein |
| NCBI Synonym Full Names: | ribonuclease, RNase A family, 3 |
| NCBI Official Symbol: | RNASE3�� |
| NCBI Official Synonym Symbols: | ECP; RNS3�� |
| NCBI Protein Information: | eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease |
| UniProt Protein Name: | Eosinophil cationic protein |
| UniProt Synonym Protein Names: | Ribonuclease 3; RNase 3 |
| Protein Family: | Eosinophil cationic protein |
| UniProt Gene Name: | RNASE3�� |
| UniProt Entry Name: | ECP_HUMAN |
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