Description
| Product Name: | Human RTCA Recombinant Protein |
| Product Code: | RPPB4533 |
| Size: | 10µg |
| Species: | Human |
| Target: | RTCA |
| Synonyms: | RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, RNA cyclase, RNA-3'-phosphate cyclase, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RTCA protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL |
RNA 3'-Terminal Phosphate Cyclase (RTCA) encodes a part of the RNA 3'-phosphate cyclase family. RTCA takes part in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. RTCA is involved in some phases of cellular RNA processing.
RTCA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 389 amino acids (1-366a.a) and having a molecular mass of 41.7kDa. RTCA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RTCD1: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'- phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.5.1.4; RNA-bindingChromosomal Location of Human Ortholog: 1p21.2Cellular Component: nucleoplasm; nucleusMolecular Function: RNA binding; RNA-3'-phosphate cyclase activity; ATP bindingBiological Process: RNA processing |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| UniProt Code: | O00442 |
| NCBI GenInfo Identifier: | 195927044 |
| NCBI Gene ID: | 8634 |
| NCBI Accession: | NP_001124313.1 |
| UniProt Secondary Accession: | O00442,Q5VVL5, Q5VVL6, Q96E99 |
| UniProt Related Accession: | O00442 |
| Molecular Weight: | 40,709 Da |
| NCBI Full Name: | RNA 3'-terminal phosphate cyclase isoform a |
| NCBI Synonym Full Names: | RNA 3'-terminal phosphate cyclase |
| NCBI Official Symbol: | RTCA�� |
| NCBI Official Synonym Symbols: | RPC; RTC1; RTCD1�� |
| NCBI Protein Information: | RNA 3'-terminal phosphate cyclase; RTC domain-containing protein 1; RNA terminal phosphate cyclase domain 1; RNA terminal phosphate cyclase domain-containing protein 1 |
| UniProt Protein Name: | RNA 3'-terminal phosphate cyclase |
| UniProt Synonym Protein Names: | RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1 |
| Protein Family: | RNA 3'-terminal phosphate cyclase |
| UniProt Gene Name: | RTCA�� |
| UniProt Entry Name: | RTCA_HUMAN |
