Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit
The Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit is a reliable and efficient tool for the precise measurement of S100A12 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.S100A12 is a key protein involved in inflammatory processes, immune response regulation, and cell differentiation.
Its levels have been implicated in various diseases, including inflammatory disorders, autoimmune diseases, and cardiovascular conditions. Monitoring S100A12 levels can provide valuable insights into disease progression and treatment efficacy, making it a valuable biomarker for research and clinical applications.
Product Name:
Human S100A12 (S100 Calcium Binding Protein A12) CLIA Kit
SKU:
HUES00745
Target:
Human S100A12 (S100 Calcium Binding Protein A12)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A12. You can calculate the concentration of Human S100A12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
98-111
90-103
Average (%)
97
105
96
1:4
Range (%)
93-108
85-96
97-112
Average (%)
99
91
105
1:8
Range (%)
93-106
86-100
102-115
Average (%)
101
92
108
1:16
Range (%)
95-110
91-102
89-100
Average (%)
102
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
98-113
105
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.79
307.75
970.14
102.81
336.89
1042.15
Standard deviation
8.5
21.88
71.5
9.58
38.74
113.8
C V (%)
8.52
7.11
7.37
9.32
11.5
10.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
