Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
The Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit is specifically designed for the accurate quantification of S100A12 levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.S100A12 is a calcium-binding protein that plays a crucial role in inflammation and immune response.
Elevated levels of S100A12 have been associated with various inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.This Human S100A12 ELISA Kit provides researchers with a reliable tool to assess S100A12 levels in biological samples, enabling further understanding of its role in disease pathology and the development of targeted treatments.
Product Name:
Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
SKU:
HUES02349
Target:
Human S100A12 (S100 Calcium Binding Protein A12)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100A12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100A12. You can calculate the concentration of Human S100A12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
99-110
91-104
Average (%)
98
104
96
1:4
Range (%)
91-104
85-100
84-96
Average (%)
98
92
89
1:8
Range (%)
89-104
81-94
87-99
Average (%)
96
87
92
1:16
Range (%)
85-100
84-95
87-99
Average (%)
92
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.08
4.12
0.48
1.06
3.86
Standard deviation
0.03
0.05
0.14
0.03
0.05
0.15
C V (%)
6.12
4.63
3.4
6.25
4.72
3.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human S100A12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
