The Human SCF (Stem Cell Factor) ELISA Kit is specifically designed for the precise measurement of stem cell factor levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees reliable and consistent results, making it an excellent choice for various research purposes.Stem cell factor, also known as c-kit ligand, is a critical cytokine involved in the regulation of stem cell proliferation, differentiation, and survival. Its role in hematopoiesis, melanogenesis, and gametogenesis makes it a valuable target for studying various diseases and developing innovative therapies. By utilizing the Human SCF ELISA Kit, researchers can effectively measure and monitor stem cell factor levels, advancing their understanding of stem cell biology and potentially identifying new treatment strategies for conditions such as cancer, blood disorders, and immune system abnormalities. Trust in the accuracy and precision of this ELISA kit for your research needs.
Product Name:
Human SCF (Stem Cell Factor) ELISA Kit
SKU:
HUES02355
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
