The Human SEMA4D (Semaphorin 4D) ELISA Kit is specifically designed for the accurate detection of SEMA4D levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it ideal for a variety of research applications.SEMA4D is a critical protein that plays a key role in immune response modulation, angiogenesis regulation, and tumor growth. It is implicated in various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions. With its reliable performance and versatility, the Human SEMA4D (Semaphorin 4D) ELISA Kit is an essential tool for researchers seeking to investigate the role of SEMA4D in disease pathology and explore its potential as a therapeutic target.
Product Name:
Human SEMA4D (Semaphorin 4D) ELISA Kit
SKU:
HUES03137
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
