Description
| Product Name: | Human SET Recombinant Protein |
| Product Code: | RPPB4652 |
| Size: | 20µg |
| Species: | Human |
| Target: | SET |
| Synonyms: | 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, Template-activating factor I, SET. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The SET solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD |
SET is part of a complex localized to the endoplasmic reticulum however is found in the nucleus. SET inhibits apoptosis following attack by cytotoxic T lymphocytes and can also enhance DNA replication of the adenovirus genome. SET inhibits acetylation of nucleosomes, particularly histone H4, by histone acetylases (HAT). This inhibition is accomplished by masking histone lysines from being acetylated.
SET Human Recombinant produced in E. coli is a single polypeptide chain containing 313 amino acids (1-290) and having a molecular mass of 35.9kDa. SET is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. |
| NCBI Summary: | The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q01105 |
| NCBI GenInfo Identifier: | 46397790 |
| NCBI Gene ID: | 6418 |
| NCBI Accession: | Q01105.3 |
| UniProt Secondary Accession: | Q01105,Q15541, Q5VXV1, Q5VXV2, Q6FHZ5, A5A5H4, A6NGV1 B4DUE2, |
| UniProt Related Accession: | Q01105 |
| Molecular Weight: | 41 kDa |
| NCBI Full Name: | Protein SET |
| NCBI Synonym Full Names: | SET nuclear proto-oncogene |
| NCBI Official Symbol: | SET�� |
| NCBI Official Synonym Symbols: | 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA�� |
| NCBI Protein Information: | protein SET |
| UniProt Protein Name: | Protein SET |
| UniProt Synonym Protein Names: | HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase; IGAAD; PHAPII; Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I |
| Protein Family: | Set3 complex |
| UniProt Gene Name: | SET�� |
