Human SIAH1 Recombinant Protein (RPPB2274)

Product Name:	Human SIAH1 Recombinant Protein
Product Code:	RPPB2274
Size:	10µg
Species:	Human
Target:	SIAH1
Synonyms:	Siah E3 ubiquitin protein ligase 1, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), E3 ubiquitin-protein ligase SIAH1, HUMSIAH, hSIAH1, Siah-1a, SIAH1A, EC 6.3.2.
Source:	Escherichia Coli

Physical Appearance:	Sterile Filtered colorless solution.
Formulation:	The SIAH1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 40% glycerol.
Stability:	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:	Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:	MGSSHHHHHH SSGLVPRGSH MGSVANSVLF PCKYASSGCE ITLPHTEKAD HEELCEFRPY SCPCPGASCK WQGSLDAVMP HLMHQHKSIT TLQGEDIVFL ATDINLPGAV DWVMMQSCFG FHFMLVLEKQ EKYDGHQQFF AIVQLIGTRK QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL FAENGNLGIN VTISMC

E3 ubiquitin-protein ligase SIAH1 (SIAH1) belongs to the SIAH (seven in absentia homolog) family. SIAH1 is a tumor suppressor protein, which is expressed in intestinal epithelium and activated during apoptosis. SIAH1 is involved in ubiquitination and proteasome-mediated degradation of specific proteins. SIAH1 is comprised of an N-terminal RING-finger domain (required for proteolysis) and a cystein-rich C-terminal domain (which regulates oligomerization and SIAH binding to target proteins). SIAH1 causes indirect degradation of beta-catenin by way of creation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi.

SIAH1 Human Recombinant produced in E. coli is a single polypeptide chain containing 216 amino acids (90-282) and having a molecular mass of 24.1 kDa.SIAH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:	SIAH1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S- nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. Homodimer. Interacts with group 1 glutamate receptors GRM1 and GRM5. Interacts with DAB1, which may inhibit its activity. Interacts with UBE2E2. Interacts with PEG3. Interacts with GAPDH; leading to stabilize SIAH1. Component of some large E3 complex composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with UBE2I. Interacts with alpha- tubulin. Interacts with PEG10, which may inhibit its activity. Interacts with KHDRBS3. Interacts with SNCAIP and HIPK2. May be induced by p53/TP53, suggesting that it may be required to modulate p53/TP53 response. The relevance of such activity in vivo is however unclear and may not exist. Widely expressed at a low level. Down- regulated in advanced hepatocellular carcinomas. Inhibited by interaction with SNCAIP (isoform 2, but not isoform 1). May be inhibited by interaction with PEG10. Belongs to the SINA (Seven in absentia) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Cell development/differentiation; EC 6.3.2.-; EC 6.3.2.19; Ligase; Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 16q12.1 Cellular Component: beta-catenin destruction complex; cytoplasm; cytosol; nucleus Molecular Function:identical protein binding; protein binding; protein C-terminus binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: anatomical structure morphogenesis; apoptosis; axon guidance; cellular protein metabolic process; nervous system development; neuron apoptosis; positive regulation of apoptosis; proteasomal ubiquitin-dependent protein catabolic process; protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; ubiquitin-dependent protein catabolic process
NCBI Summary:	This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IUQ4
NCBI GenInfo Identifier:	46577493
NCBI Gene ID:	6477
NCBI Accession:	Q8IUQ4.2
UniProt Secondary Accession:	Q8IUQ4,O43269, Q49A58, Q92880, A0FKF3,
UniProt Related Accession:	Q8IUQ4
Molecular Weight:	Observed MW: 31kDaCalculated MW: 21kDa/31kDa/34kDa
NCBI Full Name:	E3 ubiquitin-protein ligase SIAH1
NCBI Synonym Full Names:	siah E3 ubiquitin protein ligase 1
NCBI Official Symbol:	SIAH1��
NCBI Official Synonym Symbols:	SIAH1A��
NCBI Protein Information:	E3 ubiquitin-protein ligase SIAH1
UniProt Protein Name:	E3 ubiquitin-protein ligase SIAH1
UniProt Synonym Protein Names:	RING-type E3 ubiquitin transferase SIAH1Curated; Seven in absentia homolog 1; Siah-1; Siah-1a
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	SIAH1��