The Human SIGA (Secretory Immunoglobulin A) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of secretory immunoglobulin A levels in human biological samples. This CLIA kit is suitable for serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results for a variety of research applications.Secretory immunoglobulin A is an important component of the body's immune system, playing a key role in mucosal immunity and defense against pathogens.
Abnormal levels of SIGA have been associated with various diseases and conditions, making it a valuable biomarker for studying immune responses and developing targeted therapies.With its unparalleled sensitivity and specificity, the Human SIGA CLIA Kit from AssayGenie is the perfect tool for researchers looking to assess SIGA levels in human samples with confidence and accuracy. This kit is easy to use and provides rapid results, making it an essential asset for immunology research and clinical diagnostics.
Product Name:
Human sIgA (Secretory Immunoglobulin A) CLIA Kit
SKU:
HUES00731
Target:
Human sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sIgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sIgA. You can calculate the concentration of Human sIgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
96-110
87-102
Average (%)
95
102
94
1:4
Range (%)
98-112
103-118
101-112
Average (%)
103
109
107
1:8
Range (%)
98-111
101-115
85-97
Average (%)
105
107
91
1:16
Range (%)
90-105
94-110
87-97
Average (%)
97
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-96
90
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.42
605.49
1999.04
182.51
651.96
1841.97
Standard deviation
17.02
49.47
154.93
20.86
65.0
151.23
C V (%)
9.18
8.17
7.75
11.43
9.97
8.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sIgA in samples. No significant cross-reactivity or interference between Human sIgA and analogues was observed.
