Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
The Human SOD1 (Superoxide Dismutase 1) Soluble ELISA Kit is specifically designed for the accurate measurement of soluble SOD1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SOD1 is a critical antioxidant enzyme that plays a key role in neutralizing harmful free radicals in the body, protecting cells from oxidative stress and damage. Dysregulation of SOD1 has been linked to various diseases including amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders. Therefore, the measurement of SOD1 levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Human SOD1 Soluble ELISA Kit is a valuable tool for researchers studying oxidative stress-related conditions and seeking to better understand the role of SOD1 in health and disease.
Product Name:
Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
SKU:
HUES02204
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
