Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit
The Human SRD5A2 (Steroid 5 alpha-reductase 2) ELISA Kit is a reliable tool for the precise measurement of SRD5A2 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, providing consistent and accurate results for a variety of research applications.SRD5A2 is an enzyme crucial for the conversion of testosterone to dihydrotestosterone (DHT), a potent androgen involved in various physiological processes.
Dysregulation of SRD5A2 activity has been linked to conditions such as prostate cancer, male pattern baldness, and benign prostatic hyperplasia, highlighting its importance as a biomarker for studying and developing treatments for these conditions.By using the Human SRD5A2 ELISA Kit, researchers can gain valuable insights into the role of SRD5A2 in health and disease, paving the way for innovative therapeutic strategies and personalized medicine approaches.
Product Name:
Human SRD5a2 (Steroid 5 Alpha Reductase 2) ELISA Kit
SKU:
HUES03378
Target:
Human SRD5a2 (Steroid 5 Alpha Reductase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SRD5a2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SRD5a2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SRD5a2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SRD5a2. You can calculate the concentration of Human SRD5a2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
94-110
100-116
Average (%)
95
101
106
1:4
Range (%)
89-100
85-98
85-96
Average (%)
94
91
91
1:8
Range (%)
91-105
85-99
86-100
Average (%)
99
92
91
1:16
Range (%)
94-105
85-97
85-98
Average (%)
99
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
244.23
464.97
2023.42
257.44
472.85
1873.33
Standard deviation
15.87
26.08
107.24
17.66
21.99
62.94
C V (%)
6.5
5.61
5.3
6.86
4.65
3.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SRD5a2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SRD5a2 in samples. No significant cross-reactivity or interference between Human SRD5a2 and analogues was observed.
