The Human ST2 (Syntenin-2) ELISA Kit is specifically designed for the precise measurement of ST2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.ST2, also known as Syntenin-2, is a key protein involved in various biological processes, including cell adhesion, migration, and tumor progression.
It has been implicated in inflammatory responses and immune regulation, making it a crucial biomarker for studying conditions such as inflammation, autoimmune diseases, and cancer.With its high-performance features and reliability, the Human ST2 ELISA Kit is an invaluable tool for researchers exploring the role of ST2 in health and disease, paving the way for potential diagnostic and therapeutic advancements in various fields.
Product Name:
Human ST2 (Syntenin 2) ELISA Kit
SKU:
HUES02368
Target:
Human ST2 (Syntenin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ST2. You can calculate the concentration of Human ST2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
93-108
88-100
Average (%)
90
98
94
1:4
Range (%)
87-99
86-101
89-102
Average (%)
93
93
95
1:8
Range (%)
91-103
79-91
85-100
Average (%)
96
85
91
1:16
Range (%)
87-103
85-99
84-96
Average (%)
94
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
3.58
0.45
1.31
3.8
Standard deviation
0.03
0.07
0.18
0.02
0.06
0.18
C V (%)
6
5.83
5.03
4.44
4.58
4.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ST2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ST2 in samples. No significant cross-reactivity or interference between Human ST2 and analogues was observed.
