The Human Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) ELISA Kit is a specialized assay designed to quantitatively measure levels of sVEGFR-1 in various human biological samples. sVEGFR-1 plays a crucial role in regulating angiogenesis by sequestering vascular endothelial growth factor (VEGF), thereby influencing vascular development, endothelial cell function, and potentially impacting processes like tumor growth and neovascular diseases. Our sVEGFR-1 ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactured under strict quality control standards, this kit provides robust performance and user-friendly protocols, making it an ideal choice for investigating sVEGFR-1 levels and their implications in physiological and pathological conditions. Trust Assay Genie for precise quantification and in-depth analysis of sVEGFR-1 biomarkers in your studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
