Human TAF10 Recombinant Protein (RPPB2302)
- SKU:
- RPPB2302
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q12962
- Research Area:
- Enzymes
Description
| Product Name: | Human TAF10 Recombinant Protein |
| Product Code: | RPPB2302 |
| Size: | 10µg |
| Species: | Human |
| Target: | TAF10 |
| Synonyms: | TAF10 RNA Polymerase II TATA Box Binding Protein (TBP)-Associated Factor 30kDa, Transcription Initiation Factor TFIID 30 KDa Subunit, STAF28, TAF2A, TAF2H. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The TAF10 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT |
TAF10 is a small subunit of TFIID which is related to a subdivision of TFIID complexes. Studies with human and mammalian cells verified that this subunit is mandatory for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and is essential for several cellular differentiation programs.
TAF10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (84-218) and having a molecular mass of 16.9kDa.TAF10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TAF10: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF10 family. |
| UniProt Protein Details: | Protein type:Transcription initiation complex; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 11p15.3 Cellular Component: cytoplasm; nucleoplasm; nucleus; PCAF complex; perinuclear region of cytoplasm; transcription factor TFIID complex Molecular Function:enzyme binding; estrogen receptor binding; histone acetyltransferase activity; protein binding; transcription coactivator activity; transcription factor activity Biological Process: histone deubiquitination; protein homooligomerization; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12962 |
| NCBI GenInfo Identifier: | 3024688 |
| NCBI Gene ID: | 6881 |
| NCBI Accession: | Q12962.1 |
| UniProt Secondary Accession: | Q12962,O00703, Q13175, Q6FH13, |
| UniProt Related Accession: | Q12962 |
| Molecular Weight: | 21,711 Da |
| NCBI Full Name: | Transcription initiation factor TFIID subunit 10 |
| NCBI Synonym Full Names: | TATA-box binding protein associated factor 10 |
| NCBI Official Symbol: | TAF10�� |
| NCBI Official Synonym Symbols: | TAF2A; TAF2H; TAFII30�� |
| NCBI Protein Information: | transcription initiation factor TFIID subunit 10 |
| UniProt Protein Name: | Transcription initiation factor TFIID subunit 10 |
| UniProt Synonym Protein Names: | STAF28; Transcription initiation factor TFIID 30 kDa subunit; TAF(II)30; TAFII-30; TAFII30 |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | TAF10�� |
| UniProt Entry Name: | TAF10_HUMAN |
