The Human TE (Telomerase) ELISA Kit is specially designed for the precise measurement of telomerase levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.Telomerase is a key enzyme that plays a vital role in maintaining the length of telomeres, which are protective caps at the end of chromosomes. Telomerase activity is closely linked to cellular aging, cancer development, and other age-related diseases.
Therefore, detecting telomerase levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human TE (Telomerase) ELISA Kit is a valuable tool for researchers studying telomerase biology, aging, cancer, and other related fields. Its reliability and precision make it an essential instrument for advancing scientific understanding and discovery.
Product Name:
Human TE (Telomerase) ELISA Kit
SKU:
HUES01434
Target:
Human TE (Telomerase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TE. You can calculate the concentration of Human TE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
96-110
90-104
Average (%)
101
103
98
1:4
Range (%)
92-107
85-100
84-99
Average (%)
98
92
91
1:8
Range (%)
90-102
88-101
81-93
Average (%)
97
93
87
1:16
Range (%)
89-104
84-94
84-95
Average (%)
96
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.53
4.01
0.58
1.5
4.23
Standard deviation
0.03
0.07
0.2
0.04
0.08
0.15
C V (%)
5.45
4.58
4.99
6.9
5.33
3.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TE in samples. No significant cross-reactivity or interference between Human TE and analogues was observed.
