The Human TF (Tissue Factor) ELISA Kit is specifically designed for the quantitative measurement of tissue factor levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and dependable results for a variety of research purposes.Tissue factor is a key protein involved in blood coagulation and inflammation, playing a crucial role in various physiological and pathological processes.
Dysregulation of tissue factor has been linked to conditions such as thrombosis, inflammation, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its easy-to-use format and reliable performance, the Human TF ELISA Kit is an essential tool for researchers seeking to investigate the role of tissue factor in health and disease. Order yours today and take your research to the next level.
Product Name:
Human TF (Tissue Factor) ELISA Kit
SKU:
HUES01327
Target:
Human TF (Tissue Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F3/TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F3/TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F3/TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F3/TF. You can calculate the concentration of Human F3/TF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
99-112
90-105
Average (%)
103
106
96
1:4
Range (%)
90-107
99-112
85-98
Average (%)
98
106
92
1:8
Range (%)
99-110
97-113
88-98
Average (%)
104
103
93
1:16
Range (%)
98-113
101-113
90-102
Average (%)
104
107
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.1
60.96
237.83
27.43
58.54
231.58
Standard deviation
1.8
2.58
8.92
1.62
2.41
11.9
C V (%)
6.64
4.23
3.75
5.91
4.12
5.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F3/TF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F3/TF in samples. No significant cross-reactivity or interference between Human F3/TF and analogues was observed.
