Human THG1L Recombinant Protein (RPPB2317)
- SKU:
- RPPB2317
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NWX6
- Research Area:
- Enzymes
Description
| Product Name: | Human THG1L Recombinant Protein |
| Product Code: | RPPB2317 |
| Size: | 10µg |
| Species: | Human |
| Target: | THG1L |
| Synonyms: | tRNA-histidine guanylyltransferase 1-like (S. cerevisiae), Interphase cytoplasmic foci protein 45, probable tRNA(His) guanylyltransferase, induced by high glucose-1, FLJ11601, FLJ20546, ICF45, IHG-1, EC 2.7.7.79, EC 2.7.7.6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The THG1L solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAKSKFE YVRDFEADDT CLAHCWVVVR LDGRNFHRFA EKHNFAKPND SRALQLMTKC AQTVMEELED IVIAYGQSDE YSFVFKRKTN WFKRRASKFM THVASQFASS YVFYWRDYFE DQPLLYPPGF DGRVVVYPSN QTLKDYLSWR QADCHINNLY NTVFWALIQQ SGLTPVQAQG RLQGTLAADK NEILFSEFNI NYNNELPMYR KGTVLIWQKV DEVMTKEIKL PTEMEGKKMA VTRTRTKPVP LHCDIIGDAF WKEHPEILDE DS |
Probable tRNA (His) guanylyltransferase (THG1L) is a member of the tRNA (His) gyanylytransferase family. THG1L which is localized to the cytoplasm also found adjacent to the nuclear membrane and expressed in various tissues, including liver and lung. THG1L is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. THG1L adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is vital for accurate recognition of the tRNA and for the fidelity of protein synthesis. Upon DNA damage, THG1L is phosphorylated, presumably by ATM or ATR.
THG1L Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (30-298) and having a molecular mass of 34.0 kDa.THG1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ICF45: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Belongs to the tRNA(His) guanylyltransferase family.Protein type: EC 2.7.7.79; Cell cycle regulation; Mitochondrial; TransferaseChromosomal Location of Human Ortholog: 5q33.3Cellular Component: mitochondrion; cytoplasmMolecular Function: identical protein binding; tRNA guanylyltransferase activity; GTP binding; magnesium ion binding; ATP binding; tRNA bindingBiological Process: tRNA processing; tRNA modification; protein homotetramerization |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a mitochondrial protein that is induced by high levels of glucose and is associated with diabetic nephropathy. The encoded protein appears to increase mitochondrial biogenesis, which could lead to renal fibrosis. Another function of this protein is that of a guanyltransferase, adding GMP to the 5' end of tRNA(His). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q9NWX6 |
| NCBI GenInfo Identifier: | 146325755 |
| NCBI Gene ID: | 54974 |
| NCBI Accession: | Q9NWX6.2 |
| UniProt Secondary Accession: | Q9NWX6 |
| UniProt Related Accession: | Q9NWX6 |
| Molecular Weight: | |
| NCBI Full Name: | Probable tRNA(His) guanylyltransferase |
| NCBI Synonym Full Names: | tRNA-histidine guanylyltransferase 1 like |
| NCBI Official Symbol: | THG1L�� |
| NCBI Official Synonym Symbols: | ICF45; IHG-1; hTHG1�� |
| NCBI Protein Information: | probable tRNA(His) guanylyltransferase |
| UniProt Protein Name: | Probable tRNA(His) guanylyltransferase |
| UniProt Synonym Protein Names: | Interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase |
| Protein Family: | |
| UniProt Gene Name: | THG1L�� |
| UniProt Entry Name: | THG1_HUMAN |
