Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
The Human TIMP-2 (Tissue Inhibitors of Metalloproteinase-2) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of TIMP-2 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.TIMP-2 is a key player in regulating the activity of metalloproteinases, enzymes that break down extracellular matrix proteins. Dysregulation of TIMP-2 has been implicated in various diseases, including cancer, cardiovascular conditions, and inflammatory disorders. Understanding the levels of TIMP-2 in biological samples can provide valuable insights into disease mechanisms and potential therapeutic targets.Whether investigating the role of TIMP-2 in disease progression or monitoring its expression in response to treatments, the Human TIMP-2 ELISA Kit offers researchers a valuable tool for advancing their research. Trust in the accuracy and precision of this kit to uncover new insights into the complex biology of TIMP-2 and its implications for human health.
Product Name:
Human TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
SKU:
HUES02488
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
