Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
The Human TIMP-4 (Tissue Inhibitors of Metalloproteinase-4) CLIA Kit is a powerful tool for quantitative measurement of TIMP-4 levels in human tissue samples. TIMP-4 is a key regulator of extracellular matrix remodeling and plays a critical role in tissue homeostasis and repair processes.This CLIA kit offers exceptional sensitivity and accuracy, allowing researchers to accurately measure TIMP-4 levels in a variety of biological samples, including serum, plasma, and tissue lysates. The kit is easy to use and provides rapid results, making it ideal for high-throughput studies and clinical research applications.
Understanding the role of TIMP-4 in various pathological conditions, such as cancer, fibrosis, and cardiovascular diseases, is essential for developing targeted therapies and personalized treatment strategies. By accurately quantifying TIMP-4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. Unlock the potential of your research with the Human TIMP-4 CLIA Kit from Assay Genie.
Product Name:
Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
SKU:
HUES00832
Target:
Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TIMP-4. You can calculate the concentration of Human TIMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
95-109
90-102
Average (%)
96
101
97
1:4
Range (%)
83-97
100-116
83-95
Average (%)
90
106
90
1:8
Range (%)
86-99
94-107
94-110
Average (%)
93
100
102
1:16
Range (%)
103-117
89-103
99-115
Average (%)
109
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
97-115
105
Cell culture media (n=5)
91-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.57
112.76
380.28
47.95
118.58
384.23
Standard deviation
4.48
10.77
23.69
5.32
9.45
39.96
C V (%)
9.83
9.55
6.23
11.09
7.97
10.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TIMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed.
