Human Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
The Human Tissue Inhibitors of Metalloproteinase 2 (TIMP2) ELISA Kit is specifically designed for the accurate measurement of TIMP2 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable tool for various research applications.TIMP2 is a critical regulator of metalloproteinases, playing a key role in tissue remodeling, cell migration, and angiogenesis.
Dysregulation of TIMP2 has been implicated in various diseases such as cancer, cardiovascular disorders, and fibrosis, highlighting its importance as a potential biomarker for disease progression and therapeutic development.With the Human TIMP2 ELISA Kit, researchers can gain valuable insights into the role of TIMP2 in disease pathogenesis and progression, paving the way for new diagnostic and therapeutic approaches in these debilitating conditions.
Product Name:
Human Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
Product Code:
HUDL02898
Size:
96 Assays
Target:
TIMP2
Synonyms:
CSC-21K, TIMP Metallopeptidase Inhibitor 2
Detection Method:
Sandwich
Range:
46.875-3,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
