Description
| Product Name: | Human TNNI1 Recombinant Protein |
| Product Code: | RPPB4976 |
| Size: | 20µg |
| Species: | Human |
| Target: | TNNI1 |
| Synonyms: | DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TNNI1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ |
Troponin I, (TNNI1) is a member of the troponin I family. Troponin complex has 3 subunits, TNNI1 known as the inhibitory Subunit which prevents the actin-myosin interactions and thus mediating striated muscle relaxation. TNNI1 combines with tropomyosin and regulates calcium sensitivity of striated muscles by structural modifications in actin-myosin complexes.
TNNI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.8kDa.TNNI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 1q31.3 Cellular Component: troponin complex; cytosol Molecular Function:metal ion binding; actin binding Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding |
| NCBI Summary: | Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19237 |
| NCBI GenInfo Identifier: | 1351298 |
| NCBI Gene ID: | 7135 |
| NCBI Accession: | P19237.3 |
| UniProt Secondary Accession: | P19237,Q659A5, Q6FGS7, Q6FGW1, Q6ICU2, Q86T57, Q96DT9 A6NEH3, A8MSJ0, |
| UniProt Related Accession: | P19237 |
| Molecular Weight: | 187 |
| NCBI Full Name: | Troponin I, slow skeletal muscle |
| NCBI Synonym Full Names: | troponin I type 1 (skeletal, slow) |
| NCBI Official Symbol: | TNNI1�� |
| NCBI Official Synonym Symbols: | TNN1; SSTNI�� |
| NCBI Protein Information: | troponin I, slow skeletal muscle; troponin I, slow-twitch isoform |
| UniProt Protein Name: | Troponin I, slow skeletal muscle |
| UniProt Synonym Protein Names: | Troponin I, slow-twitch isoform |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNI1�� |
| UniProt Entry Name: | TNNI1_HUMAN |
