Human TOLLIP Recombinant Protein (RPPB4981)
- SKU:
- RPPB4981
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H0E2
Description
| Product Name: | Human TOLLIP Recombinant Protein |
| Product Code: | RPPB4981 |
| Size: | 10µg |
| Species: | Human |
| Target: | TOLLIP |
| Synonyms: | Toll-interacting protein, TOLLIP, IL-1RAcPIP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TOLLIP protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.2M NaCl and 2mM DTT. |
| Stability: | TOLLIP Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP |
Toll interacting protein (TOLLIP) is an inhibitory adaptor protein within Toll-like receptors. The TLR pathway is a part of the innate immune system which recognizes structurally conserved molecular patterns of microbial pathogens, initiating an inflammatory immune response. Negative regulation of TLR signaling by TOLLIP might restrict the production of proinflammatory mediators during inflammation and infection. Furthermore, TOLLIP forms a complex with Tom1 to regulate endosomal transferring of ubiquitinated proteins.
TOLLIP produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294 amino acids (1-274 a.a) and having a molecular mass of 32.4kDa.TOLLIP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family. |
| UniProt Protein Details: | Protein type:Vesicle; Inhibitor Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytoplasm; cytosol Molecular Function:kinase binding; protein binding Biological Process: cell-cell signaling; epithelial cell differentiation; phosphorylation; signal transduction |
| NCBI Summary: | This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| UniProt Code: | Q9H0E2 |
| NCBI GenInfo Identifier: | 20140803 |
| NCBI Gene ID: | 54472 |
| NCBI Accession: | Q9H0E2.1 |
| UniProt Secondary Accession: | Q9H0E2,Q9H9E6, Q9UJ69, B3KXC6, |
| UniProt Related Accession: | Q9H0E2 |
| Molecular Weight: | 22,812 Da |
| NCBI Full Name: | Toll-interacting protein |
| NCBI Synonym Full Names: | toll interacting protein |
| NCBI Official Symbol: | TOLLIP�� |
| NCBI Official Synonym Symbols: | IL-1RAcPIP�� |
| NCBI Protein Information: | toll-interacting protein |
| UniProt Protein Name: | Toll-interacting protein |
| Protein Family: | Toll-interacting protein |
| UniProt Gene Name: | TOLLIP�� |
| UniProt Entry Name: | TOLIP_HUMAN |
