The Human Total p53 (Tumor Protein p53) ELISA Kit is specifically designed for the accurate measurement of total p53 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications. Tumor Protein p53, commonly known as p53, is a key tumor suppressor protein that plays a crucial role in regulating cell cycle progression, DNA repair, and apoptosis. Dysregulation of p53 is commonly associated with cancer development and progression, making it an important biomarker for cancer research and drug development.
Overall, the Human Total p53 (Tumor Protein p53) ELISA Kit is an essential tool for researchers studying p53 biology, cancer mechanisms, and potential therapeutic interventions. With its high-quality performance and accurate results, this kit is a valuable asset for advancing scientific knowledge in the field of oncology.
Product Name:
Human Total P53 (Tumor Protein p53) ELISA Kit
SKU:
HUFI04776
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
85-88%
92-98%
85-104%
EDTA Plasma (n = 5)
89-100%
89-99%
82-101%
Heparin Plasma (n = 5)
83-97%
81-99%
80-97%
Recovery:
Sample
1
2
Serum (n = 5)
98-103
100
EDTA Plasma (n = 5)
91-101
96
Heparin Plasma (n = 5)
87-103
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
