Human TPO Recombinant Protein (RPPB1051)
- SKU:
- RPPB1051
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P40225
- Research Area:
- Cytokines
Description
| Product Name: | Human TPO Recombinant Protein |
| Product Code: | RPPB1051 |
| Size: | 10µg |
| Species: | Human |
| Target: | TPO |
| Synonyms: | MPL Ligand, THCYT1, MPLLG, TPO, ML, ThrombopoietinNirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia VirusOncogene Ligand, Megakaryocyte Growth and Development Factor, MegakaryocyteColony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte StimulatingFactor, Prepro-Thrombopoietin. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TPO protein solution (0.25mg/ml) contains phosphatebuffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSGLLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH |
Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.
TPO produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 340 amino acids (22-353aa.) andhaving a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57KDa).TPO is expressed with an 8 amino acid His tag at C-Terminusand purified by proprietary chromatographic techniques..
| UniProt Protein Function: | THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3q27 Cellular Component: extracellular space Molecular Function:growth factor activity; hormone activity; cytokine activity Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation Disease: Thrombocythemia 1 |
| NCBI Summary: | Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014] |
| UniProt Code: | P40225 |
| NCBI GenInfo Identifier: | 730982 |
| NCBI Gene ID: | 7066 |
| NCBI Accession: | P40225.1 |
| UniProt Related Accession: | P40225 |
| Molecular Weight: | |
| NCBI Full Name: | Thrombopoietin |
| NCBI Synonym Full Names: | thrombopoietin |
| NCBI Official Symbol: | THPO�� |
| NCBI Official Synonym Symbols: | ML; TPO; MGDF; MKCSF; MPLLG; THCYT1�� |
| NCBI Protein Information: | thrombopoietin |
| UniProt Protein Name: | Thrombopoietin |
| UniProt Synonym Protein Names: | C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand |
| Protein Family: | Thyroid peroxidase |
| UniProt Gene Name: | THPO�� |
| UniProt Entry Name: | TPO_HUMAN |
