Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit
The Human TRAIL (TNFSF10) ELISA Kit is specifically designed for the precise measurement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TRAIL is a critical protein that induces apoptosis in cancer cells, making it an important target for cancer therapy. Its role in immune surveillance and tumor suppression highlights its significance in cancer research and treatment. By detecting TRAIL levels, researchers can gain valuable insights into the mechanisms of cell death and potential therapeutic strategies for cancer and other diseases.
Product Name:
Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit
SKU:
HUES02602
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
97-113
91-107
95-111
Average (%)
103
98
102
1:4
Range (%)
91-101
82-94
86-96
Average (%)
96
87
91
1:8
Range (%)
95-109
82-94
89-103
Average (%)
100
87
94
1:16
Range (%)
94-107
84-99
87-99
Average (%)
100
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
87-101
93
Cell Culture Media (n=8)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.61
125.63
430.56
46.56
132.86
436.79
Standard deviation
2.23
6.47
23.47
4.02
10.22
34.24
C V (%)
4.41
5.15
5.45
8.63
7.69
7.84
Uniport ID:
P50591
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed
