Human Trypsin Pan/PRSS1-2-3 PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS1199 |
| Size: | 96T |
| Application: | This ELISA antibody pair recognizes Human Trypsin Pan. |
| Uniprot: | P07477 / P07478 / P35030 |
| Gene ID: | 5644 / 5645 / 5646 |
| Gene Names: | PRSS1 | PRSS2 | PRSS3 | PRSS4 | TRP1 | TRY1 | TRY2 | TRY3 | TRY4 | TRYP1<>|TRYP2 |
| Synonyms: | Trypsin-1 (EC 3.4.21.4) (Beta-trypsin) (Cationic trypsinogen) (Serine protease 1) (Trypsin I) [Cleaved into: Alpha-trypsin chain 1 Alpha-trypsin chain 2] / Trypsin-2 (EC 3.4.21.4) (Anionic trypsinogen) (Serine protease 2) (Trypsin II) / Trypsin-3 (EC 3.4.21.4) (Brain trypsinogen) (Mesotrypsinogen) (Serine protease 3) (Serine protease 4) (Trypsin III) (Trypsin IV) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 30.72 pg/ml - 7500 pg/ml |
| Sensitivity: | 30 pg/ml |
| Recommended Dilution: | 200 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Stop Solution |
| 4. | Assay Diluent(s) |
| 5. | Lyophilized Standard |
| 6. | Biotinylated Detection Antibody |
| 7. | Streptavidin-Conjugated HRP |
| 8. | TMB One-Step Substrate |
Other materials and equipment required:
The Assay Genie Human Trypsin Pan/PRSS1-2-3 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed in the manual. |
| 2. | Add 100 ul of standard or sample to each well. |
| 3. | Incubate 2.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul of prepared biotin antibody to each well. |
| 5. | Incubate 1 h at RT. |
| 6. | Add 100 ul of prepared Streptavidin solution to each well. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul of TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul of Stop Solution to each well. |
| 11. | Read at 450 nm immediately. |
