The Human Thrombospondin-1 (TSP-1) ELISA Kit is specifically designed for the accurate quantification of TSP-1 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TSP-1 is a critical protein involved in regulating cell adhesion, angiogenesis, and inflammation, making it a valuable biomarker for studying various health conditions such as cancer, cardiovascular diseases, and immune disorders. By accurately measuring TSP-1 levels, researchers can gain insights into the pathogenesis of these diseases and potentially discover new therapeutic targets.Overall, the Human TSP-1 ELISA Kit from Assay Genie provides researchers with a reliable and efficient tool for investigating the role of TSP-1 in disease pathophysiology and developing novel interventions for improved patient outcomes.
Product Name:
Human TSP-1 (Thrombospondin-1) ELISA Kit
SKU:
HUES02599
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
