Human TSTA3 Recombinant Protein (RPPB2354)
- SKU:
- RPPB2354
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13630
- Research Area:
- Enzymes
Description
| Product Name: | Human TSTA3 Recombinant Protein |
| Product Code: | RPPB2354 |
| Size: | 25µg |
| Species: | Human |
| Target: | TSTA3 |
| Synonyms: | FX, P35B, SDR4E1, GDP-L-fucose synthase, Protein FX . |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 0.5mg/ml solution containing 20mM Tris pH-8, 2mM DTT, 50mM NaCl & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSHMGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLAAMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSNFGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGTGNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQFKKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K |
TSTA3 is a NADP(H)-binding protein. TSTA3 catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of numerous fucosyltransferases that take part in the expression of several glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in TSTA3 result in leukocyte adhesion deficiency, type II.
TSTA3 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids (1-321 a.a.) and having a molecular mass of 38 kDa. The TSTA3 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TSTA3: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. Belongs to the fucose synthase family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.271; Carbohydrate Metabolism - amino sugar and nucleotide sugar Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytoplasm Molecular Function:GDP-L-fucose synthase activity; electron carrier activity; isomerase activity; coenzyme binding; GDP-4-dehydro-D-rhamnose reductase activity Biological Process: leukocyte adhesion; cytolysis; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process |
| NCBI Summary: | Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13630 |
| NCBI GenInfo Identifier: | 13124123 |
| NCBI Gene ID: | 7264 |
| NCBI Accession: | Q13630.1 |
| UniProt Secondary Accession: | Q13630,Q567Q9, Q9UDG7, B2R8Y7, D3DWK5, |
| UniProt Related Accession: | Q13630 |
| Molecular Weight: | 321 |
| NCBI Full Name: | GDP-L-fucose synthase |
| NCBI Synonym Full Names: | tissue specific transplantation antigen P35B |
| NCBI Official Symbol: | TSTA3�� |
| NCBI Official Synonym Symbols: | FX; P35B; SDR4E1�� |
| NCBI Protein Information: | GDP-L-fucose synthase; 3-5 epimerase/4-reductase; red cell NADP(H)-binding protein; tissue specific transplantation antigen 3; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; short chain dehydrogenase/reductase family 4E, member 1 |
| UniProt Protein Name: | GDP-L-fucose synthase |
| UniProt Synonym Protein Names: | GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; Protein FX; Red cell NADP(H)-binding protein; Short-chain dehydrogenase/reductase family 4E member 1 |
| UniProt Gene Name: | TSTA3�� |
| UniProt Entry Name: | FCL_HUMAN |
