The Human TXB2 (Thromboxane B2) ELISA Kit offered by Assaygenie is a powerful tool for accurately measuring levels of TXB2 in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. Thromboxane B2 is a critical molecule involved in platelet aggregation and vasoconstriction, playing a key role in various pathological conditions such as cardiovascular diseases, inflammation, and clot formation.
By measuring TXB2 levels using this ELISA kit, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop new therapeutic strategies. Rely on the Human TXB2 (Thromboxane B2) ELISA Kit from Assaygenie for accurate and reliable quantification of TXB2 levels, and propel your research forward in the field of cardiovascular and inflammatory diseases.
Product Name:
Human TXB2 (Thromboxane B2) ELISA Kit
SKU:
HUES03086
Target:
Human TXB2 (Thromboxane B2)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXB2. During the reaction, TXB2 in the sample or standard competes with a fixed amount of TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXB2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
97-113
96-110
Average (%)
100
105
104
1:4
Range (%)
87-101
93-107
96-114
Average (%)
92
100
104
1:8
Range (%)
90-104
90-103
93-109
Average (%)
96
96
100
1:16
Range (%)
86-98
91-108
96-111
Average (%)
93
99
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
90-107
97
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
262.7
438.8
2025.5
239.0
457.6
2206.2
Standard deviation
14.7
22.4
60.8
12.4
27.5
72.8
C V (%)
5.6
5.1
3.0
5.19
6.01
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of TXB2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes TXB2 in samples. No significant cross-reactivity or interference between TXB2 and analogues was observed.
