The Human UB (Ubiquitin) ELISA Kit is a highly precise and reliable tool for measuring ubiquitin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Ubiquitin is a vital protein involved in the regulation of protein degradation, cellular signaling, and immune response. Dysregulation of ubiquitin pathways has been linked to various diseases such as cancer, inflammatory disorders, and neurodegenerative conditions.
Therefore, the measurement of ubiquitin levels using this ELISA kit is essential for studying these diseases and developing potential therapeutic interventions.Overall, the Human UB (Ubiquitin) ELISA Kit is a valuable tool for researchers seeking to investigate the role of ubiquitin in human health and disease, providing a reliable and efficient method for detecting and quantifying ubiquitin levels in biological samples.
Product Name:
Human Ub (Ubiquitin) ELISA Kit
SKU:
HUES02315
Target:
Human Ub (Ubiquitin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Ub. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ub and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ub, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Ub. You can calculate the concentration of Human Ub in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
93-107
89-102
Average (%)
93
99
95
1:4
Range (%)
89-103
84-98
88-101
Average (%)
96
90
94
1:8
Range (%)
85-99
80-94
82-95
Average (%)
92
86
89
1:16
Range (%)
89-103
84-96
86-96
Average (%)
95
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.51
361.16
1796.23
235.84
367.38
1944.13
Standard deviation
15.03
14.92
66.28
15.52
21.27
88.85
C V (%)
6.94
4.13
3.69
6.58
5.79
4.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Ub concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ub in samples. No significant cross-reactivity or interference between Human Ub and analogues was observed.
