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Human UBE2A Recombinant Protein (RPPB2365)

SKU:
RPPB2365
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49459
Research Area:
Enzymes
€273
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Description

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Product Name:Human UBE2A Recombinant Protein
Product Code:RPPB2365
Size:25µg
Species:Human
Target:UBE2A
Synonyms:HHR6A, HR6A, RAD6A, UBC2, UBE2A, EC=6.3.2.19, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A, Ubiquitin carrier protein A, RAD6 homolog A.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:UBE2A Human solution containing 20mM Tris HCl pH-8, 1mM DTT, 1mM EDTA and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

The alteration of proteins with ubiquitin is a vital cellular mechanism for targeting atypical or short-lived proteins for degradation. Ubiquitination involves noT less than three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A is part of the E2 ubiquitin-conjugating enzyme family. UBE2A is necessary for post-replicative DNA damage repair. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is necessary for postreplication repair of UV-damaged DNA.

UBE2A Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 172 amino acids (1-152 a.a.) and having a molecular mass of 19.4 kDa. UBE2A protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

UniProt Protein Function:UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol

Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV

Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type

UniProt Code:P49459
NCBI GenInfo Identifier:33518639
NCBI Gene ID:7319
NCBI Accession:P49459.2
Molecular Weight:
NCBI Full Name:Ubiquitin-conjugating enzyme E2 A
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 A
UniProt Synonym Protein Names:RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2A��
UniProt Entry Name:UBE2A_HUMAN

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