Human UCP-2 (Uncoupling Protein 2, Mitochondrial) CLIA Kit
The Human UCP-2 (Uncoupling Protein 2) Mitochondrial CLIA Kit is a reliable tool for the quantitative measurement of UCP-2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for various research applications.UCP-2 is a mitochondrial protein that plays a vital role in cellular energy metabolism by regulating proton leak across the inner mitochondrial membrane.
Dysregulation of UCP-2 has been linked to various metabolic disorders, including obesity, diabetes, and cardiovascular diseases. The Human UCP-2 Mitochondrial CLIA Kit provides a valuable means for studying UCP-2 expression levels and understanding its implications in these diseases.Overall, the Human UCP-2 Mitochondrial CLIA Kit is a valuable tool for researchers interested in exploring the role of UCP-2 in metabolic disorders and developing potential therapeutic interventions.
Product Name:
Human UCP-2 (Uncoupling Protein 2, Mitochondrial) CLIA Kit
SKU:
HUES00859
Target:
Human UCP-2 (Uncoupling Protein 2, Mitochondrial)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UCP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UCP-2. You can calculate the concentration of Human UCP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
92-105
95-111
Average (%)
106
99
101
1:4
Range (%)
103-116
86-99
100-119
Average (%)
110
92
108
1:8
Range (%)
88-101
90-103
88-101
Average (%)
95
95
94
1:16
Range (%)
93-106
96-112
91-104
Average (%)
98
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.96
278.8
746.96
109.21
282.86
760.9
Standard deviation
11.5
24.65
64.99
11.47
20.96
52.35
C V (%)
10.75
8.84
8.7
10.5
7.41
6.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UCP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UCP-2 in samples. No significant cross-reactivity or interference between Human UCP-2 and analogues was observed.
