Human Vascular Endothelial Growth Factor 145 (VEGF145) ELISA Kit
The Human VEGF145 (Vascular Endothelial Growth Factor 145) ELISA Kit is specifically designed for the accurate and precise detection of VEGF145 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.VEGF145 is a critical protein involved in angiogenesis, the process of forming new blood vessels from pre-existing ones. It plays a key role in various physiological processes such as wound healing, embryonic development, and tumor growth.
Dysregulation of VEGF145 expression is associated with a range of diseases including cancer, diabetic retinopathy, and cardiovascular disorders.By accurately measuring VEGF145 levels, researchers can gain insights into disease progression, evaluate potential treatment outcomes, and develop novel therapeutic strategies. The Human VEGF145 ELISA Kit provides a valuable tool for studying and understanding the role of VEGF145 in health and disease.
Product Name:
Human Vascular Endothelial Growth Factor 145 (VEGF145) ELISA Kit
Product Code:
HUDL03081
Size:
96 Assays
Target:
VEGF145
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-99
95
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
