Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
The Human Vaspin ELISA Kit is a valuable tool for detecting levels of visceral adipose-specific serine protease inhibitor in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Vaspin, also known as visceral adipose-specific serine protease inhibitor, is a protein that plays a key role in regulating adipose tissue function and metabolism. Its dysregulation has been associated with obesity, insulin resistance, and other metabolic disorders, making it an important target for studying and developing therapies for these conditions. By using the Human Vaspin ELISA Kit, researchers can gain valuable insights into the role of vaspin in metabolic health and disease, advancing our understanding of obesity-related complications and potential treatment strategies. Trust in the accuracy and precision of this kit for your research needs.
Product Name:
Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
SKU:
HUES02740
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
