Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
The Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit is meticulously developed to enable the precise quantitative detection of VE-Cadherin in various human biological samples. VE-Cadherin, a critical protein involved in endothelial cell adhesion and vascular integrity, plays a significant role in regulating vascular permeability and endothelial barrier function. Accurate measurement of VE-Cadherin levels is essential for understanding vascular physiology, endothelial function, and vascular-related pathologies. Our VE-Cadherin ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results vital for in-depth research activities. Manufactained under stringent quality control standards, this kit guarantees robust performance, delivering reliable data with ease of use. Trust Assay Genie's VE-Cadherin ELISA Kit for dependable quantification of this essential vascular biomarker in your investigative studies.
Product Name:
Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
SKU:
AEES00137
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
