Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit
The Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit is specifically designed for the accurate quantification of VEGF165 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for various research applications.VEGF165 is a critical growth factor that plays a key role in angiogenesis, the process of forming new blood vessels. It is involved in various physiological and pathological processes, including wound healing, tumor growth, and cardiovascular diseases.
By measuring VEGF165 levels, researchers can gain valuable insights into these conditions and potential therapeutic interventions.Overall, the Human VEGF165 ELISA Kit is a valuable resource for studying the role of VEGF165 in various biological processes and diseases, ultimately contributing to the advancement of scientific research and clinical development.
Product Name:
Human VEGF165 (Vascular Endothelial Growth Factor165) ELISA Kit
SKU:
HUES03060
Target:
Human VEGF165 (Vascular Endothelial Growth Factor165)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF165 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF165, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF165. You can calculate the concentration of Human VEGF165 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
96-111
93-104
Average (%)
97
102
98
1:4
Range (%)
96-112
84-100
91-107
Average (%)
103
91
99
1:8
Range (%)
97-109
83-94
93-107
Average (%)
104
88
100
1:16
Range (%)
94-107
86-98
97-110
Average (%)
100
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.3
168.4
996.3
100.4
171.8
914.6
Standard deviation
6.3
7.4
30.9
5.2
7.7
43.0
C V (%)
6.47
4.39
3.1
5.18
4.48
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGF165 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF165 in samples. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
