Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
The Human VEGFR-2 (KDR) ELISA Kit is specifically designed for the precise measurement of VEGFR-2 levels in human serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring the accuracy and reproducibility of results for various research applications.VEGFR-2, also known as vascular endothelial growth factor receptor 2, plays a crucial role in angiogenesis and vascular endothelial cell function. It is a key receptor involved in the regulation of blood vessel formation and maintenance, making it an important target for studying conditions such as cancer, cardiovascular diseases, and inflammatory disorders. By accurately quantifying VEGFR-2 levels, researchers can gain valuable insights into the mechanisms underlying angiogenesis and vascular biology, paving the way for the development of novel therapeutic strategies and targeted interventions. The Human VEGFR-2 (KDR) ELISA Kit is a valuable tool for advancing research in the field of vascular biology and angiogenesis.
Product Name:
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
SKU:
HUES02611
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.