Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
The Assay Genie Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit is meticulously crafted to facilitate the quantitative detection of VEGFR1/FLT1 levels in diverse biological samples. VEGFR1, as a pivotal player in angiogenesis, vascular development, and endothelial cell function, is crucial for regulating critical processes like vascular permeability and cell survival. By accurately quantifying VEGFR1/FLT1 levels, researchers can garner valuable insights into angiogenic signaling pathways and vascular-related pathologies. Our VEGFR1/FLT1 ELISA Kit boasts outstanding sensitivity and specificity, ensuring the generation of reliable and reproducible data for your research needs. Manufactured under stringent quality control standards, this kit promises robust performance and ease of use, making it an optimal solution for conducting in-depth investigations in the field of VEGFR1-related research. Trust Assay Genie's VEGFR1/FLT1 ELISA Kit for precise and dependable quantification, empowering your scientific explorations.
Product Name:
Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
SKU:
AEES00175
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.